on Server
v4.0.3
RBC 1 and 3 trimmed (poly I:C)
RBC 2 and 4 trimmend (control)
trim parameters
Ambiguous limit = 2
Ambiguous trim = No
Custom vector trim = No
Custom vectors = []
FASTA file name =
Minimum number of nucleotides in reads = 30
Paired end data = No
Put trim-annotations on sequences = No
Quality limit = 0.05
Quality trim = Yes
Remove old trim = Yes
Show warning regarding annotations and undo = Yes
Threshold reads length = Yes
Trim sequences and save in new list = Yes
Vector limit = 1
Vector trim = No
RBC1 = 19728783
RBC2 = 16976315
RBC3 = 21269919
RBC4 = 20690534
settings
7am 5/18
running similar scenario with 4 separate RNAseq files.....